API (Analytical Profile Index) 20E presented is a biochemical panel for identification and differentiation of members of the family. Changes to thesaurus. Changes to database old new. Taxons. Notes. Identification. Additional tests. API® 20 E v v X. X. X. X. RapiD 20 E™ v v X. API® 20 Microbial Identification Kits, bioMerieux. Supplier: Quantity. API® 20 E Microbial Identification Kit, , , , Pack of 25, Retrieving.
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Using the results obtained, from each group of three reactions a 9 nine digit code number is produced. H 2 S is produced from thiosulphate. This causes a pH rise as indicated by bromothymol blue. Reading the test strip 1. The 24E solid microplate format contains the 24 substrates contained in the galegie of both the 12A and 12B strips.
Prolonged incubation, insufficient incubation, improper filling of appi, or inadequate inoculum may lead to false results.
Mix thoroughly to prepare a homogeneous suspension. Evaluate 15 to 30 minutes after the addition of reagents. Organism identification is based on pH change and substrate utilisations as established by published reference methodologies 4,6,8,9.
A Gram-stain and oxidase test should be performed prior to set-up of tests. The results should be interpreted as follows:. Green should be regarded as a negative reaction. Bromothymol blue indicator changes from blue to yellow when the carbohydrate is utilised to form acid.
All systems should be read after 48 hours for the identification of Miscellaneous Gram-negative bacilli. Ammonium released from splitting of urea causes the pH to rise – indicated by phenol red changing from yellow to pink-red. Preparation of inoculum Pick isolated colonies from an hour culture and emulsify in 2.
Thomas, Veterinary Microbiology. After the addition of Nitrate reagents A and B: The 12B strip can be used in conjunction with the 12A strip for the identification of oxidase-positive, nitrate-negative, and glucose-nonfermenters MGNB as well as the Enterobacteriaceae. Evaluate within 2 minutes of the addition of the reagent.
This will determine whether nitrate has been reduced completely to nitrogen gas N2.
Identification of Pseudomonas aeruginosa with the APIE system.
Material Safety Data Sheet. Green or blue is positive reaction. The 12A strip may be used alone for identification of oxidase-negative, nitrate-positive glucose fermenters comprising 15 genera and may be useful for screening pathogenic Enterobacteriaceae from enteric and urine specimens or identification of other common isolates. Citrate utilization citrate is the only source of carbon.
Kit presentation Each kit contains the following: Once the foil pouch has been opened, unused strips must be placed back in the foil pouch, and the foil pouch taped closed.
Each strip consists of 12 different biochemical substrates. Record all positive results. Refer to the Table of Reactions below for the substrates contained in each well, the specific reaction principle, and colour changes. The sum of these indices in each group of three reactions forms the code number. When Actinobacillus or Pasteurella spp.
Production of a red colour within a few minutes of the addition of the reagent indicates that nitrate reduction to nitrite NO 2 has occurred. Quality control The overall performance of the system should be monitored by testing appropriate control strains. Tryptophan deaminase forms indolepyruvic acid from tryptophan which produces a brown colour in the presence of ferric ions.
Acetoin is produced from glucose indicated by the formation of a pink-red complex after the addition of alpha-naphthol and creatine. The lysine and ornithine decarboxylase reactions should be carefully interpreted.
The interpretation of mathematically calculated identification results requires trained clinical personnel who should use judgement and knowledge in conjunction with the following information before accepting the ID of an organism: To determine the purity of the inoculum, it is advisable to inoculate a solid non-selective medium with the test suspension to act as a culture purity check.
Miscellaneous Gram-negative bacilli – Weakly positive reactions are recorded as negative results. A small amount of zinc powder should be added to those wells which exhibit a yellow colour after the addition of the nitrate reagents. The inclusion of a 12B strip is strongly 2e.
For aid in interpreting reactions, refer to the Table of Reactions. Record the results under the appropriate heading on the report form.
Microbact Biochemical Identification Kits. Materials Required but not provided The following materials may be required but are not provided:.
This is a negative reaction. All organisms belonging to the family Enterobacteriaceae reduce nitrates to nitrites and give a positive reaction.
Species with low frequency of occurrence require additional testing. The following galerid are recommended for independent laboratory assessment. Materials Required but not provided The following materials may be required but are not provided: